WebTK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use … WebWe studied the genotoxicity of ethyl carbamate in Salmonella typhimurium, in Saccharomyces cerevisiae and in human lymphoblastoid TK6 cells. In absence of cytochrome P450 enzymes, no ethyl carbamate-mediated genotoxicity was observed in any of the three test systems in the non-cytotoxic range. In the presence of an activating …
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WebNov 1, 2004 · Induced genomic instability in the human B lymphoblastoid cell line TK6 manifests itself as increases in end-to-end chromosome fusions and non-reciprocal chromosome translocations. It is not associated with elevated frequencies of specific locus mutations or other cytogenetic alterations. WebJul 4, 2012 · Abstract. The Organization for Economic Co-operation and Development (OECD) has recently adopted Test Guideline 487 (TG487) for conducting the in vitro micronucleus (MNvit) assay. The purpose of this study is to evaluate and validate treatment conditions for the use of p53 competent TK6 human lymphoblastoid cells in a TG487 …
WebTCL6. In molecular biology, T-cell leukemia/lymphoma 6 (non-protein coding), also known as TCL6 is a long non-coding RNA. It is expressed in T-cell leukemia with a t (14;14) … WebMar 11, 2024 · The human lymphoblastoid TK6 cell line was established in 1978 from the parent cell line WI-L2 that was derived from a 5-year-old male with hereditary spherocytosis (Lorge et al., 2016). Due to TK heterozygosity on human chromosome 17 in these cells as well as the presence of the hypoxanthine phosphoribosyltransferase ( HPRT ) gene on the …
WebOct 12, 2012 · In this report, the ethyl acetate extract and its major constituent, isorhamnetin 3-o-robinobioside (I3-O-Rob), from Nitraria retusa (N. Retusa) leaf extracts were evaluated for their ability to induce apoptosis in human lymphoblastoid cells (TK6).Apoptosis of TK6 cell line was carried out using DNA fragmentation, poly ADP-ribose polymerase (PARP) … Webthe human TK6 lymphoblastoid cell line (35), HepG2 cells (36) (37) and primary Syrian Hamster Embryo cells (38) has been described, although they have not been used in validation studies. ... cells analysed are likely to have undergone cell division during or after exposure to the test substance. For
WebJan 1, 2016 · Abstract Closely related TK6, WTK1, and NH32 human B-lymphoblastoid cell lines differ in their p53 functional status. These lines are used frequently in genotoxicity studies and in studies aimed at understanding the role of p53 in DNA repair. Despite their routine use, little is known about the genetic status of these cells.
WebApr 22, 2014 · The lymphoblastoid cell line MT1, known to be deficient for MMR ( 32 ), expressed the MMR reporter at a level up to 10-fold lower than that of the other four cell lines that have no known MMR defects. As expected, the O6 -MeG direct-reversal reporter was highly expressed in MT1 and TK6, owing to the absence of MGMT. skin assessment for diabetic patientsWebFeb 9, 2024 · Lymphoblastoid TK6 cells were treated with the genotoxins (GTX) aflatoxin B1 (AB1), benzo [ a ]pyrene (BaP), lasiocarpine (Las), methyleugenol (ME), or N-nitrosopyrrolidine (NPYR) or with non-genotoxins (NGTX) 3-monochloropropane-1,2-diol (3-MCPD), methapyrilene (MPH), perfluorooctanoic acid (PFOA), or perfluorooctanesulfonic … swamp cooler odor controlWebJan 1, 2024 · TK6, WTK1, NH32 (human lymphoblastoid cell lines) etoposide: Analysis of fixed cells: Micronuclei do not lead to apoptosis of the whole cell [54] Utani et al., 2010: HeLa (cervical cancer cell line) hydroxyurea: Live Imaging: Micronuclei lead to apoptosis of the whole cell (30%) swamp cooler odorWebFeb 20, 2024 · TK6, a human B-lymphoblastoid cell line, was received from Dr. W.J. Caspary, NIEHS, RTP, USA and was grown in RPMI 1640 medium supplemented with 10% horse … swamp cooler on roof renoWebJul 15, 2005 · To detect cell death in the human lymphoblastoid cell line TK6, sub-G 1 DNA content was measured by PI staining and flow cytometry analysis. SPER/NO induced a dose-dependent increase in the sub-G 1 population in TK6 cells, which peaked 2 days after the treatment ( Fig. 1A ). skin assessment pictureWebAug 1, 1995 · TK6 is a human cell line developed for gene mutation assays and thus may have advantages for human hazard identification and assessment. On the other hand, L5178Y, a mouse cell line, is a widely used gene mutation assay system, and its large database is helpful when assessing chemical genotoxicity and comparing magnitudes of … skin assessment tools for nursesWebATCC human lymphoblastoid tk6 cell line Human Lymphoblastoid Tk6 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more swamp cooler on solar